Invitrogen

TLR8 Polyclonal Antibody

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TLR8 Antibody (PA5-80137) in WB

Western Blot was performed using Anti-TLR8 Polyclonal Antibody (Product # PA5-80137) and a 150 kDa band corresponding to full length Toll-like receptor 8 was observed in ssRNA40 stimulated THP-1 cells but not in B-cells, which are reported negative for TLR8. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), IFN-gamma stimulated THP-1 (Lane 2), IFN-gamma stimulated THP-1 cells treated with ssRNA40 (Lane 3), Ramos (Lane 4), and Raji (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved pro... View More

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TLR8 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using TLR8 Polyclonal Antibody (Product # PA5-80137), shows expression of full length TLR8 upon stimulation with ssRNA40 in THP-1 cells and no expression in the B cell lines tested (Raji and Ramos) which are reported negative for TLR8. [doi:10.4049/jimmunol.1401375] {TM}

产品信息

PA5-80137

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of rat TLR8 (1013-1029aa ENDSRYDNLYIDSIRQY).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2747251

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

The TLR8 protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The TLR8 gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。