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TMEM129 Antibody (PA5-144022) in WB
Western blot analysis of TMEM129 using TMEM129 antibody (Product # PA5-144022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM129... View More
产品信息
PA5-144022
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human TMEM129 recombinant protein (Position: E25-R362).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3075236
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
靶标信息
Enables ubiquitin protein ligase activity. Involved in protein polyubiquitination; retrograde protein transport, ER to cytosol; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase TM129; RING-type E3 ubiquitin transferase TM129; transmembrane protein 129, E3 ubiquitin protein ligase
基因别名: D4S2561E; TMEM129
UniProt ID: (Human) A0AVI4
Entrez Gene ID: (Human) 92305
