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TMEM27 Antibody (PA5-88721) in WB
Western blot analysis of TMEM27 in extracts of various cell lines. Samples were incubated with TMEM27 Polyclonal antibody (Product # PA5-88721) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-88721
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human TMEM27 (NP_0657161)
偶联物
形式
Liquid
浓度
0.51 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805089
产品详细信息
Immunogen sequence: MLWLLFFLVT AIHAELCQPG AENAFKVRLS IRTALGDKAY AWDTNEEYLF KAMVAFSMRK VPNREATEIS HVLLCNVTQR VSFWFVVTDP SKNHTLPAVE VQSAIRMNKN RINNAFFLND QTLEFLKIPS TLAPPMDPSV PIWIIIFGVI FCIIIVAIAL LILSGIWQRR RKNKEPSEVD DAEDKCENMI TIENGIPSDP LDMKGGHIND AFMTEDERLT PL; Positive Samples: Mouse Kidney, Mouse Pancreas, Rat Kidney; Cellular Location: Membrane, Single-pass type I membrane protein
靶标信息
TMEM27 (Transmembrane protein 27) is also named as collectrin and Belongs to the TMEM27 family. It is a stimulator of beta cell replication and a membrane protein cleaved and shed by pancreatic beta cells that has been proposed as a beta cell mass biomarker. Western blot analysis of mouse collecting duct cell lines detected Tmem27 at an apparent molecular mass of 32 kDa. TMEM27 is an N-glycosylated protein and forms dimers, with MW of 64-75 kDa.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Collectrin; kidney-specific membrane protein; Transmembrane protein 27
基因别名: 0610008J07Rik; Cltrn; collectrin; NX-17; NX17; Tmem27
UniProt ID: (Rat) Q9ESG3, (Mouse) Q9ESG4
Entrez Gene ID: (Rat) 57395, (Mouse) 57394
