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TNF alpha Antibody (MP390) in IHC
Journal of inflammation (London, England) 2010 -
Figure 7 Immunohistochemical detection of TNF-alpha in biopsies of DNCB-induced rashes treated with various nanocrystalline silver-derived solutions . (A) Representative images are shown for immunohistochemical detection of TNF-alpha after 72 h treatment of negative controls with distilled water (i), and DNCB-induced porcine contact dermatitis rashes with distilled water (positive controls) (ii), or nanocry... View More
产品信息
MP390
应用
建议稀释比
已发表文章
产品规格
种属反应
Pig
已发表种属
Pig,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4F4
抗原
Recombinant porcine TNF alpha (Baculovirus-derived)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_223611
产品详细信息
MP390 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa. This antibody will not cross-react with human or mouse TNFa, mouse TNFb, or porcine TNFb.
This is a carrier-free low endotoxin formulation, <1.0 EU/mg.
This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B, 0.1-0.65 µg/mL). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080).
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
靶标信息
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor-necrosis factor
基因别名: SBAB-548A10.2; TNF; TNFA; TNFSF2
UniProt ID: (Pig) P23563
Entrez Gene ID: (Pig) 397086
