Invitrogen

TNF alpha Antibody Cocktail

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TNF alpha Antibody (AHC3712) in ELISA

The Journal of experimental medicine 1999 - Figure 3 CT treatment selectively suppressed IL-12 p70 and TNF-alpha, but not IL-10, TGF-beta 1 , or IL-6. Human monocytes (2 x 10 6 cell/mL) were stimulated with SAC (0.01%) and IFN-gamma (100 ng/mL) in the presence or absence of CT (10 ng/mL). Cytokine production was measured by ELISA 24 h after stimulation. Data are mean values +- SD of four separate experiments using four different donors. ** P <... View More

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产品信息

AHC3712

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

查看16篇已发表文章 16篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

已发表种属

Bacteria, Human

宿主/亚型

Mouse / Ig Mix

分类

Cocktail

类型

Antibody

克隆号

68B2B3 and 68B6A3

抗原

Purified recombinant human TNF-alpha.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536274

产品详细信息

This monoclonal mixture is recommended as a capture antibody in Sandwich ELISA applications.

This clone can also be referred to as clone number 68B 2B3 BC6/68B 6A3 1H8 1F2.

Clone 68B2B3 isotype is undetermined. Clone 68B6A3 isotype is IgG1.

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.

Communications biology

Rodriguez E,Boelaars K,Brown K,Madunić K,van Ee T,Dijk F,Verheij J,Li RJE,Schetters STT,Meijer LL,Le Large TYS,Driehuis E,Clevers H,Bruijns SCM,O'Toole T,van Vliet SJ,Bijlsma MF,Wuhrer M,Kazemier G,Giovannetti E,Garcia-Vallejo JJ,van Kooyk Y

AHC3712 was used in Enzyme-linked immunosorbent assay to show mechanisms by which the glyco-code contributes to the tolerogenic microenvironment in PDAC.

Tue Jan 11 00:00:00 UTC 2022

Selection of a novel and highly specific tumor necrosis factor alpha (TNFalpha) antagonist: insight from the crystal structure of the antagonist-TNFalpha complex.

The Journal of biological chemistry

Byla P,Andersen MH,Holtet TL,Jacobsen H,Munch M,Gad HH,Thøgersen HC,Hartmann R

AHC3712 was used in Functional assays to present a novel and highly specific TNFalpha antagonist developed using this technology.

Fri Apr 16 00:00:00 UTC 2010

Biocompatibility of electrochemical glucose sensors implanted in the subcutis of pigs.

Diabetes technology & therapeutics

Kvist PH,Iburg T,Bielecki M,Gerstenberg M,Buch-Rasmussen T,Hasselager E,Jensen HE

AHC3712 was used in immunohistochemistry to characterize implanted electrochemical glucose sensors in the subcutis of pigs and their biocompatibility

Tue Aug 01 00:00:00 UTC 2006

The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.

Experimental gerontology

Njemini R,Lambert M,Demanet C,Mets T

AHC3712 was used in ELISA to examine the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells

Wed Mar 01 00:00:00 UTC 2006

Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry.

Journal of biomolecular screening

Inglefield JR,Larson CJ,Gibson SJ,Lebrec H,Miller RL

AHC3712 was used in ELISA to develop an 96-well assay to quantitate DNA fragmentation in cells

Fri Sep 01 00:00:00 UTC 2006

Infliximab induces apoptosis of monocytes and T lymphocytes in a human-mouse chimeric model.

Clinical immunology (Orlando, Fla.)

Shen C,Maerten P,Geboes K,Van Assche G,Rutgeerts P,Ceuppens JL

AHC3712 was used in ELISA to investigate if caspase-dependent apoptosis induction is a mechanism of action of infliximab

Wed Jun 01 00:00:00 UTC 2005

Haptoglobin dampens endotoxin-induced inflammatory effects both in vitro and in vivo.

Immunology

Arredouani MS,Kasran A,Vanoirbeek JA,Berger FG,Baumann H,Ceuppens JL

AHC3712 was used in ELISA to examine the effect of haptoglobin on endotoxin-induced inflammatory responses

Tue Feb 01 00:00:00 UTC 2005

Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.

Journal of immunology (Baltimore, Md. : 1950)

Gorden KB,Gorski KS,Gibson SJ,Kedl RM,Kieper WC,Qiu X,Tomai MA,Alkan SS,Vasilakos JP

Tue Feb 01 00:00:00 UTC 2005

The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.

European journal of biochemistry

Vinogradov E,Paul CJ,Li J,Zhou Y,Lyle EA,Tapping RI,Kropinski AM,Perry MB

AHC3712 was used in ELISA to isolate and characterize LGLB from Spirochaeta aurantia

Wed Dec 01 00:00:00 UTC 2004

Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells.

The Journal of biological chemistry

Gee K,Kozlowski M,Kumar A

AHC3712 was used in ELISA to suggest that TNF-alpha-induced p38 MAPK activation regulates the induction of functionally active HA-binding form of CD44 by activating sialidase in LPS-stimulated human monocytic cells.

Fri Sep 26 00:00:00 UTC 2003

Euglycemic hyperinsulinemia augments the cytokine and endocrine responses to endotoxin in humans.

American journal of physiology. Endocrinology and metabolism

Soop M,Duxbury H,Agwunobi AO,Gibson JM,Hopkins SJ,Childs C,Cooper RG,Maycock P,Little RA,Carlson GL

AHC3712 was used in ELISA to examine the effect of in vivo changes in glucose availability and plasma insulin concentrations in humans.

Sat Jun 01 00:00:00 UTC 2002

Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.

Journal of immunology (Baltimore, Md. : 1950)

Gee K,Lim W,Ma W,Nandan D,Diaz-Mitoma F,Kozlowski M,Kumar A

AHC3712 was used in ELISA to study CD44 in monocytic cells.

Fri Nov 15 00:00:00 UTC 2002

Igf-I inhibits spontaneous apoptosis in human granulocytes.

Endocrinology

Kooijman R,Coppens A,Hooghe-Peters E

AHC3712 was used in ELISA to examine the effects of IGF-I on in vitro apoptosis of human peripheral blood granulocytes.

Mon Apr 01 00:00:00 UTC 2002

Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.

Blood

Braun MC,Wang JM,Lahey E,Rabin RL,Kelsall BL

AHC3712 was used in ELISA to study the mechanism of HIV-mediated suppression of IL-2 production.

Fri Jun 01 00:00:00 UTC 2001

Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.

International immunology

Bullens DM,Rafiq K,Charitidou L,Peng X,Kasran A,Warmerdam PA,Van Gool SW,Ceuppens JL

AHC3712 was used in ELISA to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines.

Thu Feb 01 00:00:00 UTC 2001

Selective suppression of IL-12 production by chemoattractants.

Journal of immunology (Baltimore, Md. : 1950)

Braun MC,Lahey E,Kelsall BL

AHC3712 was used in ELISA to determine the ability of chemoattractants to affect IL-2 production by human monocytes and dendritic cells.

Wed Mar 15 00:00:00 UTC 2000

Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides.

Journal of immunology (Baltimore, Md. : 1950)

Tapping RI,Akashi S,Miyake K,Godowski PJ,Tobias PS

AHC3712 was used in ELISA to study the contribution of TLR2 and TLR4 to LPS-induced signaling.

Wed Nov 15 00:00:00 UTC 2000

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC3712 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 UTC 1999

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC3712 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 UTC 1999

TNF alpha Antibody Cocktail

TNF alpha Antibody (AHC3712) in ELISA

产品信息

AHC3712

免疫组化 (IHC)

酶联免疫吸附实验 (ELISA)

功能检测 (Functional)

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

性能保障

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