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TNFR1 (soluble) Antibody (500-P143BT-25UG) in WB
Western Blot: To detect hsTNF-Receptor I by Western Blot analysis TNFR1 (soluble) Polyclonal Antibody, Biotin (Product # 500-P143BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P143BT-25UG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 18.3 kDa Recombinant Human TNF Receptor Type I
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929469
产品详细信息
AA Sequence of recombinant protein: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor Type I (500-P143) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I.
Western Blot: To detect hsTNF-Receptor I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P143BT-1MG will be provided as 2 x 500 µg
靶标信息
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD120a; CD120a antigen; MGC19588; n-smase activation assoc. factor; p55; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF-R1; TNF-RI; TNFR; TNFR I; TNFR-I; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; Tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
基因别名: CD120a; FPF; MS5; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR1-d2; TNFR55; TNFR60; TNFRSF1A
UniProt ID: (Human) P19438
Entrez Gene ID: (Human) 7132
prostaglandin metabolic process
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of tumor necrosis factor-mediated signaling pathway
viral process
cytokine-mediated signaling pathway
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of inflammatory response
cellular response to mechanical stimulus
death-inducing signaling complex assembly
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
positive regulation of ceramide biosynthetic process
