Invitrogen

TNIK Polyclonal Antibody

TNIK Antibody in Immunocytochemistry (ICC/IF)

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TNIK Antibody (PA1-20639) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of TNIK was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TNIK Polyclonal Antibody (Product # PA1-20639) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

TNIK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TNIK Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. 293T cells were transfected with TNIK shRNA and decrease in signal intensity was observed in Western blotting using Anti-TNIK Polyclonal Antibody (Product # PA1-20639). {KD}

产品信息

PA1-20639

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

- -

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide with a proprietary sequence

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_561781

产品详细信息

Suggested positive controls for this product are heart, skeletal muscle and brain.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

TNIK or TRAF2 and NCK interacting kinase is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function. TNIK is mainly expression in brain, heart, and spleen and it is a specific effector of RAP2 which regulate actin cytoskeleton. TNIK is autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation. TNIK has also been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。