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TOLLIP Antibody (PA5-30747) in ICC/IF
Immunofluorescence analysis of Toll-interacting protein was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TOLLIP Polyclonal Antibody (Product # PA5-30747) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (... View More
产品信息
PA5-30747
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 69 and 274 of Human Tollip
偶联物
形式
Liquid
浓度
0.63 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2548221
产品详细信息
Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, CL1-5.
Predicted reactivity: Mouse (90%), Rat (90%), Pig (88%), Chicken (87%), Rhesus Monkey (100%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: adapter protein; FLJ33531; Toll-interacting protein
基因别名: 4930403G24Rik; 4931428G15Rik; IL-1RAcPIP; TOLLIP
UniProt ID: (Human) Q9H0E2, (Mouse) Q9QZ06
Entrez Gene ID: (Human) 54472, (Mouse) 54473
