Invitrogen

TOLLIP Monoclonal Antibody (SB40a)

TOLLIP Antibody in Immunocytochemistry (ICC/IF)

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TOLLIP Antibody (MA5-28676) in ICC/IF

Immunofluorescence analysis of Toll-interacting protein was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TOLLIP Monoclonal Antibody (SB40a) (Product # MA5-28676) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More

TOLLIP Antibody in Flow Cytometry (Flow)

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产品信息

MA5-28676

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

3 µg/10^6 cells

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

SB40a

抗原

Recombinant Tollip

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

purified

保存液

BBS, pH 8.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2745635

靶标信息

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。