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TOP1 Antibody (435900) in WB
Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HCT 116 (Lane 1), Hep G2 (Lane 2), Caco-2 (Lane 3) and PC-3 (Lane 4).The blots were probed with TOP1 Mouse monoclonal Antibody (Product # 435900, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 100 kDa band corresponding to TOP1 was observed across the cell lines tested. Apart from desired band, a 70 kDa band was observed in Hep G2 cel... View More
产品信息
435900
产品规格
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
24.5
抗原
Recombinant protein derived from the N-terminal region of human TOP1 protein.
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2532213
靶标信息
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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