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TOP2B Antibody (PA5-27750) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TOP2B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
产品信息
PA5-27750
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1448 and 1626 of Human Topoisomerase II beta
偶联物
形式
Liquid
浓度
0.48 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545226
产品详细信息
Recommended positive controls: Jurkat, K562, THP-1.
Predicted reactivity: Mouse (89%), Rat (86%), Pig (88%), Rhesus Monkey (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen
基因别名: D230016L12Rik; Top-2; TOP2B; top2beta; TOPIIB
UniProt ID: (Human) Q02880, (Mouse) Q64511
Entrez Gene ID: (Human) 7155, (Mouse) 21974
chromatin binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
ATP binding
protein C-terminus binding
DNA binding, bending
enzyme binding
histone deacetylase binding
metal ion binding
protein heterodimerization activity
nucleotide binding
DNA binding
DNA topoisomerase activity
protein binding
isomerase activity
DNA metabolism protein
