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产品信息
PA1-4678
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat,
Human,
Rabbit
已发表种属
Rat
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_561855
产品详细信息
This antibody is predicted to react with wide variety of mammals based on 100% sequence homology, however it does not recognize TPH in rabbit tissues.
This antibody is specific for the ~55 kDa TPH protein in Western blots of human dorsal Raphe nucleus. Immunolabeling has been demonstrated in rat brain extracts.
靶标信息
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; Tryptophan 5-hydroxylase 1; tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)
基因别名: TPH; TPH1; TPRH; TRPH
UniProt ID: (Human) P17752, (Rabbit) P17290, (Rat) P09810
Entrez Gene ID: (Human) 7166, (Rabbit) 100101558, (Rabbit) 100009100, (Rat) 24848
circadian rhythm
aromatic amino acid family metabolic process
negative regulation of ossification
response to immobilization stress
serotonin biosynthetic process
positive regulation of fat cell differentiation
indolalkylamine biosynthetic process
bone remodeling
oxidation-reduction process
mammary gland alveolus development
serotonin biosynthetic process from tryptophan
