Invitrogen

TPH1 Polyclonal Antibody

TPH1 Antibody in Immunocytochemistry (ICC/IF)

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TPH1 Antibody (PA1-777) in ICC/IF

Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugat... View More

TPH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA1-777

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:10-1:500

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免疫沉淀 (IP)

1:1,000

产品规格

种属反应

Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the C-terminal region of TPH1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2256401

产品详细信息

PA1-777 detects TPH1 in human, mouse, rat, rabbit and non-human primate samples.

PA1-777 has been successfully used in ICC/IF, IP, and Western blot procedures. By Western blot PA1-777 detects a 51 kDa protein representing TPH1.

The PA1-777 immunogen is a synthetic peptide corresponding to the C-terminal region of TPH1.

靶标信息

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Herpes Simplex Virus-1 Induced Serotonin-Associated Metabolic Pathways Correlate With Severity of Virus- and Inflammation-Associated Ocular Disease.

Frontiers in microbiology

Battaglia DM,Sanchez-Pino MD,Nichols CD,Foster TP

Published figure using TPH1 polyclonal antibody (Product # PA1-777) in Western Blot

Sat Apr 09 00:00:00 UTC 2022

Herpes Simplex Virus-1 Induced Serotonin-Associated Metabolic Pathways Correlate With Severity of Virus- and Inflammation-Associated Ocular Disease.

Frontiers in microbiology

Battaglia DM,Sanchez-Pino MD,Nichols CD,Foster TP

Published figure using TPH1 polyclonal antibody (Product # PA1-777) in Western Blot

Sat Apr 09 00:00:00 UTC 2022

Stress inhibits tryptophan hydroxylase expression in a rat model of depression.

Oncotarget

Chen Y,Xu H,Zhu M,Liu K,Lin B,Luo R,Chen C,Li M

Fri Sep 08 00:00:00 UTC 2017

Stress inhibits tryptophan hydroxylase expression in a rat model of depression.

Oncotarget

Chen Y,Xu H,Zhu M,Liu K,Lin B,Luo R,Chen C,Li M

Fri Sep 08 00:00:00 UTC 2017

Differential tissue distribution of tryptophan hydroxylase isoforms 1 and 2 as revealed with monospecific antibodies.

Brain research

Sakowski SA,Geddes TJ,Thomas DM,Levi E,Hatfield JS,Kuhn DM

PA1-777 was used in western blot to investigate the localization of TPH isoforms in various tissues

Wed Apr 26 00:00:00 UTC 2006

生物信息学

蛋白别名: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)

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基因别名: TPH; TPH1; TPH2; TPRH; TRPH