Invitrogen

TPH2 Polyclonal Antibody

TPH2 Antibody in Immunoprecipitation (IP)

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TPH2 Antibody (PA1-778) in IP

Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (Product # PA1-778). Product # PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody ... View More

TPH2 Antibody in Immunohistochemistry (IHC)

TPH2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

TPH2 Antibody — Figure 1 Polymerase chain reaction and immunocytochemistry results. (A) Expression of the Cre (left) and TPH2 (right) marker genes. (B) Immunofluorescence in 5-HT-positive neurons in the wild-type group (magnification, x20). (C) 5-HT-positive neurons were lost in the TPH2-knockout group (magnification, x20). Scale bar=20 mum. Mlf, medial longitudinal fasciculus; 5-HT, 5-hydroxytryptamine; TPH2, tryptophan hydroxylase 2. {KO}

产品信息

PA1-778

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:500

免疫沉淀 (IP)

1:1,000

其他PubMed (MISC)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2207687

产品详细信息

PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples.

PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2.

The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.

靶标信息

Tryptophan hydroxylase (TPH) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. TPH is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Sex-specific modulation of the medial prefrontal cortex by glutamatergic median raphe neurons.

Science advances

Collins SA,Stinson HE,Himes A,Nestor-Kalinoski A,Ninan I

PA1-778 was used in Immunohistochemistry (Frozen) to demonstrate a cell type-specific modulation of the mPFC by MRN (VGLUT3) neurons and reveal a sex-specific role of this neuromodulation in mPFC synaptic plasticity.

Fri Nov 10 00:00:00 UTC 2023

The Mesoscopic Connectome of the Cholinergic Pontomesencephalic Tegmentum.

Frontiers in neuroanatomy

Zhao P,Wang H,Li A,Sun Q,Jiang T,Li X,Gong H

PA1-778 was used in Immunohistochemistry to reveal the integrated cholinergic connectome of the midbrain, thus improving the present understanding of the organizational structure of the pontine-tegmental cholinergic system from its anatomical structure to its functional modulation.

Sat Jul 16 00:00:00 UTC 2022

Depletion of TrkB Receptors From Adult Serotonergic Neurons Increases Brain Serotonin Levels, Enhances Energy Metabolism and Impairs Learning and Memory.

Frontiers in molecular neuroscience

Sahu MP,Pazos-Boubeta Y,Steinzeig A,Kaurinkoski K,Palmisano M,Borowecki O,Piepponen TP,Castrén E

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry (PFA fixed)

Tue May 04 00:00:00 UTC 2021

Sexually dimorphic response of colorectal motility to noxious stimuli in the colorectum in rats.

The Journal of physiology

Horii K,Ehara Y,Shiina T,Naitou K,Nakamori H,Horii Y,Shimaoka H,Saito S,Shimizu Y

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout mice.

Journal of neurochemistry

Haase J,Jones AKC,Mc Veigh CJ,Brown E,Clarke G,Ahnert-Hilger G

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Western Blot

Fri Oct 01 00:00:00 UTC 2021

Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout mice.

Journal of neurochemistry

Haase J,Jones AKC,Mc Veigh CJ,Brown E,Clarke G,Ahnert-Hilger G

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Western Blot

Fri Oct 01 00:00:00 UTC 2021

Serotonergic Plasticity in the Dorsal Raphe Nucleus Characterizes Susceptibility and Resilience to Anhedonia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Prakash N,Stark CJ,Keisler MN,Luo L,Der-Avakian A,Dulcis D

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Wed Jan 15 00:00:00 UTC 2020

Serotonergic Plasticity in the Dorsal Raphe Nucleus Characterizes Susceptibility and Resilience to Anhedonia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Prakash N,Stark CJ,Keisler MN,Luo L,Der-Avakian A,Dulcis D

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Wed Jan 15 00:00:00 UTC 2020

Combined exposure to dinotefuran and chronic mild stress counteracts the change of the emotional and monoaminergic neuronal activity induced by either exposure singly despite corticosterone elevation in mice.

The Journal of veterinary medical science

Takada T,Yoneda N,Hirano T,Onaru K,Mantani Y,Yokoyama T,Kitagawa H,Tabuchi Y,Nimako C,Ishizuka M,Ikenaka Y,Hoshi N

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Tue Mar 24 00:00:00 UTC 2020

The Journal of veterinary medical science

Takada T,Yoneda N,Hirano T,Onaru K,Mantani Y,Yokoyama T,Kitagawa H,Tabuchi Y,Nimako C,Ishizuka M,Ikenaka Y,Hoshi N

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Tue Mar 24 00:00:00 UTC 2020

Differential role of dose and environment in initiating and intensifying neurotoxicity caused by MDMA in rats.

BMC pharmacology & toxicology

Shokry IM,Shields CJ,Callanan JJ,Ma Z,Tao R

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Mon Aug 05 00:00:00 UTC 2019

Protection of tissue physicochemical properties using polyfunctional crosslinkers.

Nature biotechnology

Park YG,Sohn CH,Chen R,McCue M,Yun DH,Drummond GT,Ku T,Evans NB,Oak HC,Trieu W,Choi H,Jin X,Lilascharoen V,Wang J,Truttmann MC,Qi HW,Ploegh HL,Golub TR,Chen SC,Frosch MP,Kulik HJ,Lim BK,Chung K

PA1-778 was used in Immunohistochemistry-immunofluorescence to demonstrate rapid three-dimensional phenotyping of core needle biopsies and human brain cells.

Mon Dec 17 00:00:00 UTC 2018

Motherhood and infant contact regulate neuroplasticity in the serotonergic midbrain dorsal raphe.

Psychoneuroendocrinology

Holschbach MA,Lonstein JS

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Western Blot

Wed Feb 01 00:00:00 UTC 2017

Associations among within-litter differences in early mothering received and later emotional behaviors, mothering, and cortical tryptophan hydroxylase-2 expression in female laboratory rats.

Hormones and behavior

Ragan CM,Harding KM,Lonstein JS

PA1-778 was used in western blot to study how variability in maternal care received among female rat siblings affects the siblings' later emotional and maternal behaviors