Invitrogen

TPM1 Polyclonal Antibody

TPM1 Antibody in Immunocytochemistry (ICC/IF)

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TPM1 Antibody (PA5-29846) in ICC/IF

Immunofluorescence analysis of TPM1 was performed using 70% confluent log phase HSkMs cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TPM1 Rabbit Polyclonal Antibody (Product # PA5-29846) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

TPM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TPM1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of TPM1 was observed in U-2 OS and A549 in comparison to K562, HL-60 and Raji using TPM1 Polyclonal Antibody (Product # PA5-29846) in western blot. {RE}

产品信息

PA5-29846

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

Assay-dependent

产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 92 and 273 of Human Tropomyosin 1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547320

产品详细信息

Recommended positive controls: A431, mouse liver, rat muscle.

Predicted reactivity: Mouse (99%), Rat (88%), Zebrafish (80%), Xenopus laevis (83%), Pig (88%), Rabbit (88%), Chicken (81%), Sheep (88%), Bovine (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue.

Stem cell reports

Shen S,Sewanan LR,Shao S,Halder SS,Stankey P,Li X,Campbell SG

PA5-29846 was used in Western Blotting to present a method whereby maturation of hiPSC-CMs can be accelerated by simultaneous application of physiological Ca2+ and frequency-ramped electrical pacing in culture.

Tue Sep 13 00:00:00 UTC 2022

Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates.

Scientific reports

Leni G,Tedeschi T,Faccini A,Pratesi F,Folli C,Puxeddu I,Migliorini P,Gianotten N,Jacobs J,Depraetere S,Caligiani A,Sforza S

PA5-29846 was used in Western Blotting to assess the allergenicity of black soldier fly and lesser mealworm.

Mon Jan 27 00:00:00 UTC 2020

miRNA‑183‑5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1.

Oncology reports

Lin J,Shen J,Yue H,Cao Z

Published figure using TPM1 polyclonal antibody (Product # PA5-29846) in Western Blot

Sun Dec 01 00:00:00 UTC 2019

生物信息学

蛋白别名: alpha tropomyosin; Alpha-tropomyosin; cardiomyopathy, hypertrophic 3; epididymis secretory protein Li 265; hepatoma alpha tropomyosin; sarcomeric tropomyosin kappa; smooth muscle alpha-tropomyosin; striated muscle alpha-tropomyosin; TPM1-alpha; tropomyosin; tropomyosin 1 (alpha); tropomyosin 3 alpha; Tropomyosin alpha-1 chain; Tropomyosin-1

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基因别名: AA986836; AI854628; Alpha-tm; C15orf13; CMD1Y; CMH3; HEL-S-265; HTM-alpha; LVNC9; TM2; Tm3; Tma2; Tmpa; TMSA; Tpm-1; TPM1; Tpma