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TRH Antibody (PA5-97006) in WB
Western blot analysis of TRH in extracts of various cell lines. Samples were incubated with TRH Polyclonal antibody (Product # PA5-97006) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-97006
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 25-230 of human TRH (NP_0090481)
偶联物
形式
Liquid
浓度
0.75 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808808
产品详细信息
Positive Samples: SH-SY5Y, Mouse brain
Immunogen sequence: QPEAAQQEAV TAAEHPGLDD FLRQVERLLF LRENIQRLQG DQGEHSASQI FQSDWLSKRQ HPGKREEEEE EGVEEEEEEE GGAVGPHKRQ HPGRREDEAS WSVDVTQHKR QHPGRRSPWL AYAVPKRQHP GRRLADPKAQ RSWEEEEEEE EREEDLMPEK RQHPGKRALG GPCGPQGAYG QAGLLLGLLD DLSRSQGAEE KRQHPG
靶标信息
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Pro-thyrotropin-releasing hormone; Pro-TRH; Prothyroliberin; protirelin; thyrotropin releasing hormone; thyrotropin-releasing factor; thyrotropin-releasing hormone; TSH-releasing factor
基因别名: Pro-TRH; TRF; TRH
UniProt ID: (Human) P20396, (Mouse) Q62361
Entrez Gene ID: (Human) 7200, (Mouse) 22044
response to hypoxia
histamine metabolic process
signal transduction
cell-cell signaling
adult walking behavior
response to cold
response to glucose
hormone-mediated signaling pathway
negative regulation of glutamate secretion
positive regulation of gamma-aminobutyric acid secretion
positive regulation of insulin secretion
eating behavior
response to ethanol
response to corticosterone
negative regulation of feeding behavior
