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TRIB3 Antibody (PA5-75683) in ICC/IF
Immunofluorescence analysis of Tribbles homolog 3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with TRIB3 Polyclonal Antibody (Product # PA5-75683) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More
产品信息
PA5-75683
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues in Human TRIB3.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2719411
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
A novel inhibitor of cell division, Tribbles, blocks mitosis at a critical point in Drosophila morphogenesis. Recently, mammalian homologues of Tribbles (Trbs) have been identified that control mitogen-activated protein kinase (MAPK) activity. TRIB3 also known as TRB-3, SINK, and SKIP3 is a member of Trbs family. Overexpression of this protein inhibits NF-kB-dependent transcription induced by tumor necrosis factor (TNF) stimulation or its downstream signaling proteins but does not inhibit NF-kB translocation to the nucleus and binding to DNA. TRIB3/SKIP3 is also overexpressed in multiple human tumors and is regulated by hypoxia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DJ1103G7.3; induced in fatty liver dystrophy 2; kinase; Neuronal cell death Inducible Putative Kinase; Neuronal cell death-inducible putative kinase; p65-interacting inhibitor of NF-kappa-B; p65-interacting inhibitor of NF-kappaB; SINK; TRB-3; Tribbles 3; Tribbles homolog 3
基因别名: C20orf97; Ifld2; NIPK; SINK; SKIP3; TRB-3; TRB3; TRIB3
UniProt ID: (Human) Q96RU7, (Mouse) Q8K4K2, (Rat) Q9WTQ6
Entrez Gene ID: (Human) 57761, (Mouse) 228775, (Rat) 246273
transcription corepressor activity
protein kinase inhibitor activity
protein binding
ATP binding
kinase activity
protein kinase binding
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
ubiquitin-protein transferase regulator activity
protein kinase activity
transferase activity, transferring phosphorus-containing groups
enzyme binding
non-receptor serine/threonine protein kinase
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
protein phosphorylation
negative regulation of protein kinase activity
regulation of glucose transport
positive regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to insulin stimulus
response to endoplasmic reticulum stress
regulation of MAP kinase activity
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
negative regulation of transcription, DNA-templated
positive regulation of ubiquitin-protein transferase activity
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
regulation of transcription, DNA-templated
apoptotic process
positive regulation of protein catabolic process
negative regulation of nucleic acid-templated transcription
programmed cell death
