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TRIF Antibody (PA5-95078) in ICC/IF
Immunofluorescence analysis of TIR domain-containing adapter molecule 1 was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TRIF Polyclonal Antibody (Product # PA5-95078) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More
产品信息
PA5-95078
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human TRIF (692-712aa NNHMWNQRGSQAPEDKTQEAE).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2806884
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hCG_39321; HSD34; MyD88-3; Proline-rich, vinculin and TIR domain-containing protein B; Putative NF-kappa-B-activating protein 502H; RNF36; TI; TICAM-1; TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adapter protein inducing IFN-beta; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; toll-like receptor adaptor molecule 1
基因别名: IIAE6; MyD88-3; PRVTIRB; TICAM-1; TICAM1; TRIF
UniProt ID: (Human) Q8IUC6
Entrez Gene ID: (Human) 148022
macrophage activation involved in immune response
MyD88-independent toll-like receptor signaling pathway
inflammatory response
I-kappaB kinase/NF-kappaB signaling
positive regulation of B cell proliferation
positive regulation of protein ubiquitination
lipopolysaccharide-mediated signaling pathway
positive regulation of protein binding
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
negative regulation of MyD88-independent toll-like receptor signaling pathway
TRIF-dependent toll-like receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
regulation of protein homodimerization activity
positive regulation of chemokine biosynthetic process
innate immune response
positive regulation of interferon-beta biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of NF-kappaB transcription factor activity
defense response to virus
necroptotic process
apoptotic signaling pathway
