Invitrogen

TRIM15 Polyclonal Antibody

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2 篇参考文献

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TRIM15 Antibody in Immunohistochemistry (IHC)

TRIM15 Antibody in Immunohistochemistry (IHC)

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TRIM15 Antibody (PA5-40946) in IHC

Immunohistochemistry analysis of human intestine tissue using an anti-TRIM15 polyclonal antibody (Product # PA5-40946).

TRIM15 Antibody in Immunohistochemistry (IHC)

TRIM15 Antibody in Immunohistochemistry (IHC)

TRIM15 Antibody in Western Blot (WB)

TRIM15 Antibody in Western Blot (WB)

TRIM15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

TRIM15 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

TRIM15 Antibody in Western Blot (WB)

Product Image Gallery

TRIM15 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human intestine tissue using an anti-TRIM15 polyclonal antibody (Product # PA5-40946).

TRIM15 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human kidney tissue using an anti-TRIM15 polyclonal antibody (Product # PA5-40946).

TRIM15 Antibody in Western Blot (WB) — Western blot analysis of human HepG2 cell lysate using an anti-TRIM15 polyclonal antibody (Product # PA5-40946).

TRIM15 Antibody in Western Blot (WB) — Fig. 6 TRIM15-mediated Nrf2 signaling regulates growth and invasion in NSCLC cells in vitro. A Western blot analyses of TRIM15, Nrf2, Keap1, and Nrf2 target NQO1 in H1299 cells with TRIM15 knockdown with or without subsequent Nrf2 overexpression and H1650 cells overexpressing TRIM15 with or without subsequent knockdown of Nrf2. B ARE Luc reporter activity assessed in H1299 cells expressing shTRIM15, sh TRIM15 + Nrf2 or H1650 cells overexpressing TRIM15 with or without subsequent knockdown of Nrf2. Up-regulating of Nrf2 expression in H1299 cells or down-regulating of Nrf2 expression in H1650 cells was set as a control. C - F Cell proliferation ( C , D ), invasion ( E ) and ROS formation ( F ) in H1299 cells with or without shTRIM15 and Nrf2 rescue or in H1650 cells with or without TRIM15 overexpression and shNrf2 rescue. Statistical analyses were performed by two-tailed unpaired Student's t -test. ** P < 0.01

TRIM15 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 7 TRIM15 mediated increase in Nrf2 regulates growth and invasion in vivo. A Nude mice were randomized into three groups and subcutaneously injected with H1650 cells that had been transfected with control (empty vector), TRIM15, or TRIM15 + shNrf2 plasmids. Tumors formed in nude mice were collected 30 days after grafting, and the tumor weight were measured. B Measurement of tumor volume in experimental groups over time. C Western blotting analysis was performed to evaluate the levels of TRIM15, Nrf2, Keap1, and NQO1 in harvested tumors. D , E Up-regulation of TRIM15 significantly promoted lung metastasis in H1650 xenograft nude mice models, whereas the suppression of Nrf2 prevented the tumor metastasis of TRIM15 overexpressing cells. Representative pictures of the lung metastases in nude mice by H&E staining. Quantification of lung metastases in all groups. Scale bar: 200 mum. F , G A representative image of tumor growth in nude mice subcutaneously inoculated with H1299 cells tranfected with shCtrl, shTRIM15 or shTRIM15 + Nrf2 plasmids. Tumor volumes were measured on the indicated days. H Western blotting analysis was performed to evaluate the levels of TRIM15, Nrf2, Keap1, and NQO1 in xenograft tumors. I Representative pictures of the lung metastases in nude mice by H&E staining. Quantification of lung metastases in all groups. Scale bar: 200 mum. J TRIM15 was significantly upregulated in NSCLC and that increased TRIM15 was associated with poor survival. TRIM15 promoted

TRIM15 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 1 TRIM15 is upregulated in NSCLC and associated with NSCLC progression. A The gene expression of TRIM15 between NSCLC samples and normal lung tissue from GEPIA. LUAD (Lung adenocarcinoma), LUSC (Lung squamous cell carcinoma). B Western blot analysis of TRIM15 expression in NSCLC tissues compared with corresponding noncancerous lung tissues. C IHC analysis of TRIM15 expression in 2 NSCLC samples. Representative images are shown. Scale bar, 100 mum. D Representative IHC images of TRIM15 in NSCLC tissues with or without distant metastasis. Scale bar, 200 mum. E OS analysis of patients with NSCLC stratified by the TRIM15 expression level in 98 samples. Patients with high-expression levels of TRIM15 had shorter OS times than patients with low expression levels. ** P < 0.01

TRIM15 Antibody in Western Blot (WB) — Roles of TRIM15 in promoting NSCLC growth and metastasis. A Expression levels of TRIM15 in the indicated lung cancer cell lines were analysed by western blotting. Confirmation of TRIM15 knockdown in H1299. H1650 cells were transfected with control plasmids or vector expressing TRIM15 or TRIM15-DeltaRING. Immunoblot detection of TRIM15 and TRIM15-DeltaRING expression in H1650 cells. B , C The effect of TRIM15 knockdown and overexpression on NSCLC cells proliferation was assessed by CCK8 and EdU-incorporation assay. D , E The effects of TRIM15 loss- or gain-of-function on migration ( D ) and invasion ( E ) of NSCLC cells. Knockdown of TRIM15 resulted in significant inhibited migration ( D ) and invasion ( E ) of H1299 cells. TRIM15 upregulation significantly increased cancer cell migration ( D ) and invasion ( E ), while the TRIM15-DeltaRING lost this ability. Statistical analyses were performed by two-tailed unpaired Student's t -test. ** P < 0.01

产品信息

PA5-40946

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1.25 µg/mL

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免疫组化 (IHC)

5 µg/mL

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the N-terminal of human TRIM15 (aa 141-190).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2605607

产品详细信息

Peptide sequence: RDEIEDVKCQ EDQKLQVLLT QIESKKHQVE TAFERLQQEL EQQRCLLLAR

Sequence homology: Cow: 86%; Horse: 86%; Human: 100%; Mouse: 93%; Rabbit: 79%; Rat: 86%

靶标信息

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

E3 ligase TRIM15 facilitates non-small cell lung cancer progression through mediating Keap1-Nrf2 signaling pathway.

Cell communication and signaling : CCS

Liang M,Wang L,Sun Z,Chen X,Wang H,Qin L,Zhao W,Geng B

Published figure using TRIM15 polyclonal antibody (Product # PA5-40946) in Immunohistochemistry

Mon May 09 00:00:00 UTC 2022

E3 ligase TRIM15 facilitates non-small cell lung cancer progression through mediating Keap1-Nrf2 signaling pathway.

Cell communication and signaling : CCS

Liang M,Wang L,Sun Z,Chen X,Wang H,Qin L,Zhao W,Geng B

Published figure using TRIM15 polyclonal antibody (Product # PA5-40946) in Immunohistochemistry

Mon May 09 00:00:00 UTC 2022

生物信息学

蛋白别名: E3 ubiquitin-protein ligase TRIM15; RING finger protein 93; tripartite motif-containing 15; Zinc finger protein 178; Zinc finger protein B7

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基因别名: RNF93; TRIM15; ZNF178; ZNFB7

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UniProt ID: (Human) Q9C019

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Entrez Gene ID: (Human) 89870

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protein binding zinc ion binding ubiquitin-protein ligase

mesodermal cell fate determination positive regulation of type I interferon production innate immune response positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of RIG-I signaling pathway negative regulation of intracellular transport of viral material negative regulation of viral release from host cell