Invitrogen

TRIM23 Polyclonal Antibody

TRIM23 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

TRIM23 Antibody (PA5-34624) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of TRIM23 using TRIM23 Polyclonal Antibody (Product # PA5-34624). Samples used were HCT116 cells mock (left) and cells treated with 30 µM Cisplatin for 24 hrs (right) fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 Polyclonal Antibody (Product # PA5-34624) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.

TRIM23 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRIM23 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

PA5-34624

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 1 and 44 of TRIM23 (Uniprot ID#P36406)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.67 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2551976

产品详细信息

Recommended positive controls: HeLaS3.

Predicted reactivity: Rhesus Monkey (100%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Whole exome sequencing reveals the maintained polyclonal nature from primary to metastatic malignant peripheral nerve sheath tumor in two patients with NF1.

Neuro-oncology advances

Godec A,Jayasinghe R,Chrisinger JSA,Prudner B,Ball T,Wang Y,Srihari D,Kaushal M,Dietz H,Zhang X,Pekmezci M,Dahiya S,Tao Y,Luo J,Van Tine BA,Ding L,Gutmann DH,Hirbe AC

PA5-34624 was used in Immunohistochemistry to demonstrate the shared clonal origins of metastatic lesions relative to the primary tumors.

Wed Jul 01 00:00:00 UTC 2020

生物信息学

蛋白别名: ADP-ribosylation factor domain protein 1, 64kD; ADP-ribosylation factor domain protein 1, 64kDa; ADP-ribosylation factor domain-containing protein 1; ARF domain protein 1; E3 ubiquitin-protein ligase TRIM23; GTP-binding protein ARD-1; RING finger protein 46; RING-type E3 ubiquitin transferase TRIM23; tripartite motif protein 23; tripartite motif protein TRIM23; tripartite motif-containing 23; Tripartite motif-containing protein 23

查看更多收起

基因别名: 6330516O20Rik; AI450195; ARD1; ARFD1; RNF46; TRIM23

查看更多收起

UniProt ID: (Human) P36406

查看更多收起

Entrez Gene ID: (Human) 373, (Mouse) 81003

查看更多收起

功能