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TRIM25 Antibody (MA5-31936) in ICC/IF
Immunocytochemistry analysis of TRIM25 in Hela cells (green). Sample was incubated with TRIM25 monoclonal antibody (Product # MA5-31936) using a dilution of 1:50-1:250 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
产品信息
MA5-31936
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
5B5B10
抗原
Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2787559
产品详细信息
MA5-31936 has been tested in indirect ELISA.
靶标信息
TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; tripartite motif protein 25; tripartite motif protein TRIM25; tripartite motif-containing 25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147
基因别名: AA960166; AL022677; EFP; RNF147; TRIM25; Z147; Zfp147; ZNF147
UniProt ID: (Human) Q14258, (Mouse) Q61510
Entrez Gene ID: (Human) 7706, (Mouse) 217069
transcription factor activity, sequence-specific DNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
acid-amino acid ligase activity
poly(A) RNA binding
cadherin binding involved in cell-cell adhesion
ubiquitin protein ligase activity involved in ERAD pathway
ligase activity
metal ion binding
immune system process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of viral entry into host cell
negative regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
defense response to virus
regulation of viral release from host cell
negative regulation of viral release from host cell
protein monoubiquitination
translesion synthesis
negative regulation of type I interferon production
ERAD pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
response to estrogen
interferon-gamma-mediated signaling pathway
cell-cell adhesion
