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TRIM5 beta Antibody (PA5-120537) in WB
Western blot analysis of TRIM5 beta in extracts of various cell lines. Samples were incubated with TRIM5 beta Polyclonal antibody (Product # PA5-120537) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-120537
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2).
偶联物
形式
Liquid
浓度
1.21 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914109
产品详细信息
Positive test controls include: HeLa, SGC-7901, Mouse stomach. The target is usually found in the following locations: Cytoplasm, Nucleus, P-body.
Immunogen sequence: EKLLLFCQED GKVICWLCER SQEHRGHHTF LTEEVAREYQ VKLQAALEML RQKQQEAEEL EADIREEKAS WKTQIQYDKT NVLADFEQLR DILDWEESNE L
靶标信息
The protein encoded by this gene is a member of the tripartite motif family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coiled-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiquitinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: RING finger protein 88; RING-type E3 ubiquitin transferase TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa; tripartite motif protein TRIM5; Tripartite motif-containing protein 5
基因别名: EG667823; Gm8833; RGD1304579; RNF88; TRIM5; TRIM5alpha
UniProt ID: (Human) Q9C035
Entrez Gene ID: (Human) 85363, (Mouse) 667823, (Rat) 308906
activation of innate immune response
pattern recognition receptor signaling pathway
autophagy
regulation of lipopolysaccharide-mediated signaling pathway
regulation of protein localization
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
innate immune response
negative regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
defense response to virus
interferon-gamma-mediated signaling pathway
protein K63-linked ubiquitination
negative regulation of viral release from host cell
protein trimerization
