Invitrogen

TRPA1 Polyclonal Antibody

TRPA1 Antibody in Immunocytochemistry (ICC/IF)

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TRPA1 Antibody (PA1-46159) in ICC/IF

Immunocytochemistry analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159).

TRPA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA1-46159

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫组化（石蜡） (IHC (P))

1:100-1:250

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

Assay-Dependent

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Guinea pig, Human, Mouse, Rat, Zebrafish

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the N-terminus (residues 1-100) of the human TRPA1 protein.[Swis-Prot #075762]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2209939

产品详细信息

The target sequence has 69% sequence homology with rat.

Suggested positive control: human brain membrane fraction.

靶标信息

TRPA1 is a TRP-related channel that responds to cold temperatures and pungent compounds and plays a role in both nociceptor and hair cell transduction. TRPA1 is a transformation -associated gene product in lung epithelia, whereas its protein distribution is primarily restricted to sensory neurons. Blocking TRPA1 may be a therapeutic target for treating cold hyperalgesia caused by inflammation and nerve damage. It is now known that TRPA1 protein is also widely expressed outside of the CNS and is dys-regulated during oncogenic transformation. The structure of TRPA1 is highly related to both the protein ankyrin and transmembrane proteins. The specific function of TRPA1 has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transient receptor potential ankyrin 1 (TRPA1) mediates reactive oxygen species-induced Ca²⁺ entry, mitochondrial dysfunction, and caspase-3/7 activation in primary cultures of metastatic colorectal carcinoma cells.

Cell death discovery

Faris P,Rumolo A,Pellavio G,Tanzi M,Vismara M,Berra-Romani R,Gerbino A,Corallo S,Pedrazzoli P,Laforenza U,Montagna D,Moccia F

PA1-46159 was used in Western Blotting to find that TRPA1 channel protein is up-regulated and mediates enhanced hydrogen peroxide (H2O2)-induced Ca2+ entry in mCRC cells as compared to non-neoplastic control cells.

Sat Jul 01 00:00:00 UTC 2023

Cell death discovery

Faris P,Rumolo A,Pellavio G,Tanzi M,Vismara M,Berra-Romani R,Gerbino A,Corallo S,Pedrazzoli P,Laforenza U,Montagna D,Moccia F

Sat Jul 01 00:00:00 UTC 2023

Allyl Isothiocianate Induces Ca²⁺ Signals and Nitric Oxide Release by Inducing Reactive Oxygen Species Production in the Human Cerebrovascular Endothelial Cell Line hCMEC/D3.

Cells

Berra-Romani R,Brunetti V,Pellavio G,Soda T,Laforenza U,Scarpellino G,Moccia F

PA1-46159 was used in Western Blotting to suggest that AITC could be exploited to restore NO signalling and restore CBF in brain disorders that feature neurovascular dysfunction.

Tue Jun 27 00:00:00 UTC 2023

Allyl Isothiocianate Induces Ca²⁺ Signals and Nitric Oxide Release by Inducing Reactive Oxygen Species Production in the Human Cerebrovascular Endothelial Cell Line hCMEC/D3.

Cells

Berra-Romani R,Brunetti V,Pellavio G,Soda T,Laforenza U,Scarpellino G,Moccia F

PA1-46159 was used in Western Blotting to suggest that AITC could be exploited to restore NO signalling and restore CBF in brain disorders that feature neurovascular dysfunction.

Tue Jun 27 00:00:00 UTC 2023

Interleukin 1 beta-induced calcium signaling via TRPA1 channels promotes mitogen-activated protein kinase-dependent mesangial cell proliferation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Soni H,Kumar R,Kanthakumar P,Adebiyi A

PA1-46159 was used in Western Blotting to show that the beta isoform of IL-1 (IL-1β) is a potent inducer of IL-1 type I receptor-dependent Ca2+ entry in mouse Glomerular mesangial cell.

Thu Jul 01 00:00:00 UTC 2021

Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.

Scientific reports

Cojocaru F,Şelescu T,Domocoş D,Măruţescu L,Chiritoiu G,Chelaru NR,Dima S,Mihăilescu D,Babes A,Cucu D

PA1-46159 was used in Western Blotting to support the idea of channel-dependent and independent role for TRPA1 in tumoral processes.

Thu Jan 21 00:00:00 UTC 2021

Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.

Scientific reports

Cojocaru F,Şelescu T,Domocoş D,Măruţescu L,Chiritoiu G,Chelaru NR,Dima S,Mihăilescu D,Babes A,Cucu D

PA1-46159 was used in Western Blotting to support the idea of channel-dependent and independent role for TRPA1 in tumoral processes.

Thu Jan 21 00:00:00 UTC 2021

Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice.

The Journal of experimental biology

Wen J,Bo T,Zhang X,Wang Z,Wang D

PA1-46159 was used in Western Blotting to test the hypothesis that thermo-TRPs and gut microbiota are involved in reducing diet-induced obesity (DIO) during low temperature exposure.

Thu Jun 04 00:00:00 UTC 2020

生物信息学

蛋白别名: ANKTM1; ankyrin-like with transmembrane domains 1; Ankyrin-like with transmembrane domains protein 1; ion channel; drTRPA1; nompC; p120; Transformation-sensitive protein p120; transient receptor potential A1; Transient receptor potential cation channel subfamily A member 1; transient receptor potential cation channel, subfamily A, member 1; TRPA-1; Wasabi receptor

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基因别名: ANKTM1; FEPS; TRPA1; TRPA1b

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UniProt ID: (Human) O75762, (Mouse) Q8BLA8, (Rat) Q6RI86