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TRPV3 Antibody (MA5-45456) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TrpV3 monoclonal antibody (Product # MA5-45456) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 antibody (D) Composite.
产品信息
MA5-45456
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
N15/39
抗原
Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931910
产品详细信息
1 µg/mL of MA5-45456 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody|Detects approximately 70kDa.
靶标信息
TRPV3 belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: heat sensitive channel TRPV3; MGC124324; MGC124325; non-hair; Transient receptor potential cation channel subfamily V member 3; transient receptor potential cation channel, subfamily V, member 3; TRP ion channel TRPV3; TrpV3; Vanilloid receptor-like 3; vanilloid receptor-related osmotically activated channel protein; VRL 3; VRL-3
基因别名: 1110036I10Rik; AI644701; FNEPPK2; Nh; OLMS; TRPV3; VRL3
UniProt ID: (Human) Q8NET8, (Mouse) Q8K424
Entrez Gene ID: (Human) 162514, (Mouse) 246788, (Rat) 497948
