Invitrogen

TSPO Polyclonal Antibody

TSPO Antibody in Immunocytochemistry (ICC/IF)

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TSPO Antibody (PA5-75544) in ICC/IF

Immunofluorescence analysis of TSPO was performed using THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSPO Recombinant Rabbit Monoclonal Antibody (Product # PA5-75544) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000... View More

TSPO Antibody in Immunohistochemistry (IHC)

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TSPO Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of TSPO was observed in THP1, Raw 264.7 in comparison to SK-O-V3, HeLa and HepG2 using TSPO Polyclonal Antibody (Product # PA5-75544) in Western Blot. {RE}

产品信息

PA5-75544

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to amino acids 124-169 of Human TSPO.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2719272

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activation of translocator protein alleviates mechanical allodynia and bladder dysfunction in cyclophosphamide-induced cystitis through repression of BDNF-mediated neuroinflammation.

European journal of pain (London, England)

Huang Y,Su M,Zhang C,Zhan H,Yang F,Gao Z,Zhou X,Liu B

PA5-75544 was used in Immunohistochemistry to explore whether activation of TSPO attenuates mechanical allodynia and bladder dysfunction by regulating BDNF induced neuroinflammation in a CYP-induced cystitis model.

Fri Jul 01 00:00:00 UTC 2022

Activation of translocator protein alleviates mechanical allodynia and bladder dysfunction in cyclophosphamide-induced cystitis through repression of BDNF-mediated neuroinflammation.

European journal of pain (London, England)

Huang Y,Su M,Zhang C,Zhan H,Yang F,Gao Z,Zhou X,Liu B

Fri Jul 01 00:00:00 UTC 2022

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.

Cells

González-Blanco L,Bermejo-Millo JC,Oliveira G,Potes Y,Antuña E,Menéndez-Valle I,Vega-Naredo I,Coto-Montes A,Caballero B

PA5-75544 was used in Western Blotting to study changes in cell morphology, cell proliferation, cell death, the cell cycle, mitochondrial functionality, and the levels of pluripotency and neurogenesis of P19 stem cells treated with the TSPO ligand, PK 11195, in comparison to differentiation induced by retinoid acid (RA) and undifferentiated P19 stem cells.

Sun Oct 17 00:00:00 UTC 2021

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.

Cells

González-Blanco L,Bermejo-Millo JC,Oliveira G,Potes Y,Antuña E,Menéndez-Valle I,Vega-Naredo I,Coto-Montes A,Caballero B

Sun Oct 17 00:00:00 UTC 2021

Amyloid and Tau Induce Cell Death Independently of TSPO Polymerization and Density Changes.

ACS omega

Tournier BB,Ceyzériat K,Bouteldja FN,Millet P

PA5-75544 was used in Western Blotting to determine the effects of Aβ, Tau, and TSPO antagonists on proliferation/cell death and TSPO polymerization in the C6 astrocytic cell line.

Tue Jul 27 00:00:00 UTC 2021

TSPO ligands prevent the proliferation of vascular smooth muscle cells and attenuate neointima formation through AMPK activation.

Acta pharmacologica Sinica

Wu LP,Gong ZF,Wang H,Zhou ZS,Zhang MM,Liu C,Ren HM,Yang J,Han Y,Zeng CY

PA5-75544 was used in Western Blotting to investigate the role of endogenous TSPO in neointima formation after angioplasty in vitro and in vivo.

Wed Jan 01 00:00:00 UTC 2020

TSPO ligands prevent the proliferation of vascular smooth muscle cells and attenuate neointima formation through AMPK activation.

Acta pharmacologica Sinica

Wu LP,Gong ZF,Wang H,Zhou ZS,Zhang MM,Liu C,Ren HM,Yang J,Han Y,Zeng CY

PA5-75544 was used in Western Blotting to investigate the role of endogenous TSPO in neointima formation after angioplasty in vitro and in vivo.

Wed Jan 01 00:00:00 UTC 2020

Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Tong J,Williams B,Rusjan PM,Mizrahi R,Lacapère JJ,McCluskey T,Furukawa Y,Guttman M,Ang LC,Boileau I,Meyer JH,Kish SJ

PA5-75544 was used in Western Blotting to show some similarities between TSPO protein and PET binding characteristics in the human brain but also suggest that part of the TSPO protein pool might be less available for radioligand binding.

Fri May 01 00:00:00 UTC 2020

Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Tong J,Williams B,Rusjan PM,Mizrahi R,Lacapère JJ,McCluskey T,Furukawa Y,Guttman M,Ang LC,Boileau I,Meyer JH,Kish SJ

Fri May 01 00:00:00 UTC 2020

The peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone, ameliorates neurofunctional and neuroinflammatory abnormalities in a rat model of Gulf War Illness.

PloS one

Keledjian K,Tsymbalyuk O,Semick S,Moyer M,Negoita S,Kim K,Ivanova S,Gerzanich V,Simard JM

PA5-75544 was used in Immunohistochemistry (PFA fixed) to evaluate prophylactic treatment with the PPARγ agonist, rosiglitazone (ROSI), using a a novel model of Gulf War (GW) Illness.

Mon Jan 04 00:00:00 UTC 2021

The peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone, ameliorates neurofunctional and neuroinflammatory abnormalities in a rat model of Gulf War Illness.

PloS one

Keledjian K,Tsymbalyuk O,Semick S,Moyer M,Negoita S,Kim K,Ivanova S,Gerzanich V,Simard JM

PA5-75544 was used in Immunohistochemistry (PFA fixed) to evaluate prophylactic treatment with the PPARγ agonist, rosiglitazone (ROSI), using a a novel model of Gulf War (GW) Illness.

Mon Jan 04 00:00:00 UTC 2021

生物信息学

蛋白别名: Benzodiazepin receptor (peripheral); benzodiazepine peripheral binding site; benzodiazepine receptor, peripheral; isoquinoline-binding protein; Mitochondrial benzodiazepine receptor; OTTHUMP00000028891; PBR; Peripheral-type benzodiazepine receptor; PKBS; Translocator protein; translocator protein (18kDa)

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基因别名: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; TSPO; Tspo1

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UniProt ID: (Human) P30536, (Rat) P16257, (Mouse) P50637

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Entrez Gene ID: (Human) 706, (Rat) 24230, (Mouse) 12257

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功能

androgen binding protein binding benzodiazepine receptor activity cholesterol binding ion channel binding

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