Invitrogen

TSPO Recombinant Rabbit Monoclonal Antibody (SA90-03)

TSPO Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

TSPO Antibody (MA5-31966) in ICC/IF

Immunofluorescence analysis of TSPO was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TSPO Recombinant Rabbit Monoclonal Antibody (SA90-03) (Product # MA5-31966) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More

TSPO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TSPO Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of TSPO was observed in THP1, Raw 264.7, HeLa, MCF7, Caki 1, PC3 in comparison to SK-O-V3 and HepG2 using Anti-TSPO Recombinant Rabbit Monoclonal Antibody (SA90-03) (Product # MA5-31966) in Western Blot. {RE}

产品信息

MA5-31966

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:400-1:1000

免疫细胞化学 (ICC/IF)

1:1000

流式细胞分析 (Flow)

1:1000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SA90-03

抗原

Synthetic peptide within Human PBR aa 120-169

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809260

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: benzodiazepine peripheral binding site; benzodiazepine receptor, peripheral; isoquinoline-binding protein; Mitochondrial benzodiazepine receptor; OTTHUMP00000028891; PBR; Peripheral-type benzodiazepine receptor; PKBS; Translocator protein; translocator protein (18kDa)

查看更多收起

基因别名: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR; TSPO; Tspo1

查看更多收起

UniProt ID: (Human) P30536, (Mouse) P50637

查看更多收起

Entrez Gene ID: (Human) 706, (Mouse) 12257

查看更多收起

功能

androgen binding protein binding benzodiazepine receptor activity cholesterol binding ion channel binding

参与通路