Invitrogen

TTK Monoclonal Antibody (N1)

TTK Antibody in Immunocytochemistry (ICC/IF)

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TTK Antibody (35-9100) in ICC/IF

Immunofluorescence analysis of Dual specificity protein kinase TTK was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TTK Monoclonal Antibody (N1) (Product # 35-9100) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Ale... View More

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TTK Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Dual specificity protein kinase TTK siRNA and loss of signal was observed in Western Blot application using Anti-TTK Monoclonal Antibody (N1) (Product # 35-9100). {KD}

产品信息

35-9100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

2 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Human, Rat, Tag

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

抗原

His6-tagged human Mps1 recombinant protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533230

靶标信息

TTK is a serine/threonine kinase closely related to SPK1, PIM 1 and CDC2 serine/threonine kinases. TTK is a dual-specificity kinase, which can also phosphorylate tyrosine. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. TTK encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

High Proliferation Rate and a Compromised Spindle Assembly Checkpoint Confers Sensitivity to the MPS1 Inhibitor BOS172722 in Triple-Negative Breast Cancers.

Molecular cancer therapeutics

Anderhub SJ,Mak GW,Gurden MD,Faisal A,Drosopoulos K,Walsh K,Woodward HL,Innocenti P,Westwood IM,Naud S,Hayes A,Theofani E,Filosto S,Saville H,Burke R,van Montfort RLM,Raynaud FI,Blagg J,Hoelder S,Eccles SA,Linardopoulos S

35-9100 was used in Immunoprecipitation to study BOS172722 inhibition of spindle assembly checkpoint induced by paclitaxel in human tumor xenograft models of TNBC, as measured by inhibition of the phosphorylation of histone H3 and the phosphorylation of the MPS1 substrate, KNL1.

Tue Oct 01 00:00:00 UTC 2019

Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy.

British journal of cancer

Faisal A,Mak GWY,Gurden MD,Xavier CPR,Anderhub SJ,Innocenti P,Westwood IM,Naud S,Hayes A,Box G,Valenti MR,De Haven Brandon AK,O'Fee L,Schmitt J,Woodward HL,Burke R,vanMontfort RLM,Blagg J,Raynaud FI,Eccles SA,Hoelder S,Linardopoulos S

35-9100 was used in Immunocytochemistry-immunoflourescence to evaluate the cellular potency of a selective and potent MPS1 inhibitor, and the rate of inhibition required for therapeutic anti-tumour efficacy.

Tue Apr 25 00:00:00 UTC 2017

Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy.

British journal of cancer

Tue Apr 25 00:00:00 UTC 2017

Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).

Journal of medicinal chemistry

Naud S,Westwood IM,Faisal A,Sheldrake P,Bavetsias V,Atrash B,Cheung KM,Liu M,Hayes A,Schmitt J,Wood A,Choi V,Boxall K,Mak G,Gurden M,Valenti M,de Haven Brandon A,Henley A,Baker R,McAndrew C,Matijssen B,Burke R,Hoelder S,Eccles SA,Raynaud FI,Linardopoulos S,van Montfort RL,Blagg J

Fri Dec 27 00:00:00 UTC 2013

Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).

Journal of medicinal chemistry

Fri Dec 27 00:00:00 UTC 2013

Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).

Journal of medicinal chemistry

Fri Dec 27 00:00:00 UTC 2013

High frequency of TTK mutations in microsatellite-unstable colorectal cancer and evaluation of their effect on spindle assembly checkpoint.

Carcinogenesis

Niittymäki I,Gylfe A,Laine L,Laakso M,Lehtonen HJ,Kondelin J,Tolvanen J,Nousiainen K,Pouwels J,Järvinen H,Nuorva K,Mecklin JP,Mäkinen M,Ristimäki A,Ørntoft TF,Hautaniemi S,Karhu A,Kallio MJ,Aaltonen LA

35-9100 was used in immunohistochemistry - paraffin section to characterize nonsense-mediated decay-escaping frameshift mutations in microsatellite-unstable colorectal cancers.

Tue Mar 01 00:00:00 UTC 2011

Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression.

Proceedings of the National Academy of Sciences of the United States of America

Fisk HA,Mattison CP,Winey M

Tue Dec 09 00:00:00 UTC 2003

生物信息学

蛋白别名: cancer/testis antigen 96; Dual specificity protein kinase TTK; monopolar spindle 1 kinase; monopolar spindle 1-like 1; OTTHUMP00000016778; OTTHUMP00000220743; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT

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基因别名: CT96; ESK; MPH1; MPS1; MPS1L1; PYT; TTK

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UniProt ID: (Human) P33981

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Entrez Gene ID: (Human) 7272

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功能

protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity protein binding ATP binding non-receptor serine/threonine protein kinase

参与通路

spindle organization mitotic spindle organization mitotic spindle assembly checkpoint positive regulation of cell proliferation positive regulation of pathway-restricted SMAD protein phosphorylation peptidyl-tyrosine phosphorylation chromosome separation