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TWIST2 Antibody (PA5-99393) in ICC/IF
Immunofluorescent analysis of TWIST2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TWIST2 polyclonal antibody (Product # PA5-99393) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-99393
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TWIST2(Accession Q8WVJ9), corresponding to amino acid residues S10-S60.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818326
产品详细信息
Antibody detects endogenous levels of total Twist2.
靶标信息
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bHLHa39; Class A basic helix-loop-helix protein 39; dermis expressed 1; Dermis-expressed protein 1; Dermo-1; dermo-1 protein; twist basic helix-loop-helix transcription factor 2; twist homolog 2; twist-related bHLH protein Dermo1; Twist-related protein 2
基因别名: AMS; BBRSAY; BHLHA39; DERMO1; FFDD3; SETLSS; TWIST2
UniProt ID: (Human) Q8WVJ9, (Mouse) Q9D030, (Rat) P97831
Entrez Gene ID: (Human) 117581, (Mouse) 13345, (Rat) 59327
DNA binding
protein binding
protein dimerization activity
transcription factor activity, RNA polymerase II transcription factor binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein domain specific binding
molecular_function
basic helix-loop-helix transcription factor
transcription, DNA-templated
multicellular organism development
cell differentiation
negative regulation of apoptotic process
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
negative regulation of cell proliferation
positive regulation of keratinocyte proliferation
negative regulation of tumor necrosis factor production
negative regulation of DNA binding
negative regulation of molecular function
negative regulation of myeloid cell differentiation
embryonic cranial skeleton morphogenesis
face morphogenesis
cornea development in camera-type eye
