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TXK Antibody (PA5-99845) in ICC/IF
Immunofluorescent analysis of TXK in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TXK polyclonal antibody (Product # PA5-99845) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-99845
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TXK(Accession P42681), corresponding to amino acid residues I30-V80.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818778
产品详细信息
Antibody detects endogenous levels of total TXK.
靶标信息
TXK, along with the close relatives BMS, ITK, and BTK, signal downstream of antigen receptors and other types of receptors. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Protein-tyrosine kinase 4; PTK-RL-18; PTK4 protein tyrosine kinase 4; Resting lymphocyte kinase; tyrosine kinase; Tyrosine-protein kinase TXK
基因别名: A130089B16Rik; BTKL; PSCTK5; PTK-RL-18; PTK4; RLK; TKL; TXK
UniProt ID: (Human) P42681, (Mouse) P42682
Entrez Gene ID: (Human) 7294, (Mouse) 22165
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II regulatory region DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
nucleotide binding
DNA binding
protein kinase activity
protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
cytokine production
adaptive immune response
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
activation of phospholipase C activity
integrin-mediated signaling pathway
regulation of platelet activation
cell differentiation
positive regulation of interferon-gamma production
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
tissue regeneration
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
T cell receptor signaling pathway
positive regulation of interferon-gamma-mediated signaling pathway
NK T cell differentiation
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
phosphorylation
interferon-gamma production
interleukin-4 production
innate immune response
