Invitrogen

Talin Monoclonal Antibody (TA205)

Talin Antibody in Immunocytochemistry (ICC/IF)

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Talin Antibody (MA5-28133) in ICC/IF

Immunofluorescence analysis of Talin 1 was performed using MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Talin 1 Goat Polyclonal Antibody (Product # MA5-28133) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000... View More

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Talin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells lines tested owing to their inherent genetic constitution. Relative expression of Talin 1 was observed in MDA-MB-231 as compared to MCF7 which is reported to be low to negative for Talin 1 expression using Talin 1 Antibody (Product # MA5-28133) in western blot analysis. {RE}

产品信息

MA5-28133

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1:25-1:100

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

TA205

抗原

Human platelet talin

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2745116

产品详细信息

Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a approximately 225 kDa cytoskeletal protein.

靶标信息

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The interaction of β-arrestin1 with talin1 driven by endothelin A receptor as a feature of α5β1 integrin activation in high-grade serous ovarian cancer.

Cell death & disease

Masi I,Ottavi F,Del Rio D,Caprara V,Vastarelli C,Giannitelli SM,Fianco G,Mozetic P,Buttarelli M,Ferrandina G,Scambia G,Gallo D,Rainer A,Bagnato A,Spadaro F,Rosanò L

MA5-28133 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to highlight a new role of ETAR/β-arr1 operating an inside-out integrin activation to modulate the metastatic process and suggest that in the new integrin-targeting programs might be considered that ETAR/β-arr1 regulates Intα5β1 functional pathway.