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Tankyrase Antibody (PA5-98206) in ICC/IF
Immunofluorescent analysis of Tankyrase in HepG2 cells using a Tankyrase polyclonal antibody (Product # PA5-98206) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells w... View More
Product Details
PA5-98206
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Tankyrase-1 protein (35-130AA)
Conjugate
Form
Liquid
Concentration
3.66 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2812819
Target Information
Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:10988299, PubMed:11739745, PubMed:16076287, PubMed:19759537, PubMed:21478859, PubMed:22864114, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed:22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed:10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed:16076287). Stimulates 26S proteasome activity (PubMed:23622245). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosyltransferase diphtheria toxin-like 5; ARTD5; HGNC:11941; Poly [ADP-ribose] polymerase 5A; Poly [ADP-ribose] polymerase tankyrase-1; Protein poly-ADP-ribosyltransferase tankyrase-1; TANK1; Tankyrase I; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase; Tankyrase-1; TNKS-1; TRF1-interacting ankyrin-related ADP-ribose polymerase; TRF1-interacting ankyrin-related ADP-ribose polymerase 1
Gene Aliases: ARTD5; PARP-5a; PARP5A; PARPL; pART5; TIN1; TINF1; TNKS; TNKS1
UniProt ID: (Human) O95271
Entrez Gene ID: (Human) 8658
protein polyubiquitination
protein ADP-ribosylation
mitotic spindle organization
mitotic nuclear division
protein transport
Wnt signaling pathway
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
regulation of telomere maintenance via telomerase
positive regulation of telomere maintenance via telomerase
positive regulation of transcription from RNA polymerase II promoter
mRNA transport
spindle assembly
cell division
positive regulation of telomerase activity
protein localization to chromosome, telomeric region
protein poly-ADP-ribosylation
protein auto-ADP-ribosylation
positive regulation of canonical Wnt signaling pathway
positive regulation of telomere capping
negative regulation of telomere maintenance via telomere lengthening
negative regulation of telomeric DNA binding
negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric
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