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Tapasin Antibody (PA5-102535) in ICC/IF
Immunocytochemistry analysis of Tapasin in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Tapasin polyclonal antibody (Product # PA5-102535) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102535
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TAPBP(Accession O15533), corresponding to amino acid residues P111-V161.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851937
Product Specific Information
Antibody detects endogenous levels of total TAPBP.
Target Information
This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DADB-159G18.7; NGS-17; TAP-associated protein; TAP-binding protein; Tapasin; TPN
Gene Aliases: NGS17; TAPA; TAPBP; TPN; TPSN
UniProt ID: (Human) O15533
Entrez Gene ID: (Human) 6892, (Rat) 25217
antigen processing and presentation of peptide antigen via MHC class I
protein complex assembly
retrograde vesicle-mediated transport, Golgi to ER
immune response
regulation of gene expression
antigen processing and presentation of endogenous peptide antigen via MHC class I
peptide antigen stabilization
transmembrane transport
regulation of protein complex stability
MHC class I protein complex assembly
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
defense response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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