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Tapasin Antibody (PA5-96016) in IHC (P)
Immunohistochemistry analysis of Tapasin in paraffin-embedded human gastric cancer. Samples were incubated with Tapasin Polyclonal antibody (Product # PA5-96016) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-96016
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_7573452)
Conjugate
Form
Liquid
Concentration
2.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807818
Product Specific Information
Immunogen sequence: VEDASGKGLA KRPGALLLRQ GPGEPPPRPD LDPELYLSVH DPAGALQAAF RRYPRGAPAP HCEMSRFVPL PASAKWASGL TPAQNCPRAL DGAWLMVSIS SPVLSLSSLL RPQPEPQQEP VLITMATVVL TVLTHTPAPR VRLGQDALLD LSFAYMPPTS EAASSLAPGP PPFGLEWRRQ HLGKGHLLLA ATPGLNGQMP AAQEGAVAFA AWDDDEPWGP WTGNGTFWLP T; Positive Samples: HT-29; Cellular Location: Endoplasmic reticulum membrane, Single-pass type I membrane protein
Target Information
This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DADB-159G18.7; NGS-17; TAP-associated protein; TAP-binding protein; Tapasin; TPN; TPSN
Gene Aliases: D17Wsu91e; NGS17; TAPA; TAPBP; TPN; TPSN
UniProt ID: (Human) O15533, (Mouse) Q9R233
Entrez Gene ID: (Human) 6892, (Rat) 25217, (Mouse) 21356
antigen processing and presentation of peptide antigen via MHC class I
protein complex assembly
retrograde vesicle-mediated transport, Golgi to ER
immune response
regulation of gene expression
antigen processing and presentation of endogenous peptide antigen via MHC class I
peptide antigen stabilization
transmembrane transport
regulation of protein complex stability
MHC class I protein complex assembly
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
defense response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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