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Telomerase catalytic subunit Polyclonal Antibody

Telomerase catalytic subunit Antibody in Immunocytochemistry (ICC/IF)

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Telomerase catalytic subunit Antibody (600-401-252) in ICC/IF

Immunofluorescence microscopy of Saos-2 cells transduced with a retroviral vector expressing hTERT and green fluorescent protein (GFP) from an internal ribosomal entry site (IRES). Panel A shows native GFP expression (green), Panel B shows DAPI staining of chromosomes (blue), Panel C shows anti-hTERT staining at a 1:500 dilution followed by washes and addition of a 1:1000 dilution of rhodamine Goat anti-Rab... View More

Telomerase catalytic subunit Antibody in Western Blot (WB)

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产品信息

600-401-252

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

免疫组化 (IHC)

1:500

免疫细胞化学 (ICC/IF)

1:500

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

免疫沉淀 (IP)

2 µL/mg

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of hTERT (accession number AF018167).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Although it has been reported that this antibody reacts with mouse TERT (mTERT) (see Drissi, et al. 2001), the binding to mTERT is considerably weaker and less specific than the binding to hTERT (not shown).

靶标信息

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。