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ThPOK Antibody (12-5928-82) in Flow
C57Bl/6 thymocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD8a FITC (Product # 11-0081-82), followed by intracellular staining with 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.125 µg of Anti-Mouse ThPOK PE using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). The histogram demonstrates ThPOK staining of CD4 SP (purple histogram) and CD8 SP (orange histogram) thymocytes.
产品信息
12-5928-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
2POK
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11149862
产品详细信息
Description: This 2POK monoclonal antibody reacts with mouse ThPOK, which is also known as c-Krox and Zbtb7b. In hematopoietic cells, this 78 kDa transcription factor is expressed in thymocytes and T lymphocytes in the spleen and lymph nodes. In non-hematopoietic cells, ThPOK can be detected in the skin, heart, smooth muscle, and liver. Studies demonstrate that ThPOK promotes CD4+ lineage differentiation by inhibiting the expression of CD8 lineage-specific genes such as Runx3. ThPOK has also been shown to inhibit Cd4 silencing, another key facet required for differentiation to the CD4+ lineage. In addition to its role in thymocyte development, ThPOK is involved in the effector functions of invariant natural killer T (iNKT) cells and gamma delta T cells.
Applications Reported: This 2POK antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 2POK antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
ZFP67 is an early growth response gene that encodes a zinc finger-containing transcription factor that binds to the promoter regions of type I collagen genes (e.g., COL1A1; MIM 120150) and has a role in development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: c-Krox; DKFZp686G01254; Krueppel-related zinc finger protein cKrox; RP11-307C12.9; T helper-inducing POZ/Krueppel factor; T-helper-inducing POZ/Krueppel-like factor; Vzinc finger and BTB domain containing 7B; Zfp-67; Zinc finger and BTB domain-containing protein 7B; Zinc finger protein 67; Zinc finger protein Th-POK
基因别名: c-Krox; Thpok; Zbtb7b; Zfp67
UniProt ID: (Mouse) Q64321
Entrez Gene ID: (Mouse) 22724
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein homodimerization activity
metal ion binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
positive regulation of gene expression
cell differentiation
regulation of CD4-positive, alpha-beta T cell differentiation
regulation of CD8-positive, alpha-beta T cell differentiation
positive regulation of transcription from RNA polymerase II promoter
