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Thrombopoietin Antibody (PA5-47666) in Neu
Neutralization of Thrombopoietin in MO7e human megakaryocytic leukemic cell line. Samples were incubated in Thrombopoietin polyclonal antibody (Product # PA5-47666). Recombinant Mouse Thrombopoietin/Tpo stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti‚Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody. The ... View More
产品信息
PA5-47666
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin/Tpo and Sf 21-derived recombinant mouse Thrombopoietin/Tpo
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2608847
产品详细信息
This antibody neutralizes the biological activity of recombinant mouse Thrombopoietin/Tpo. This antibody will also neutralize the activity of recombinant human (rh) Thrombopoietin/Tpo, although 25 times the amount of Ig is required. In direct ELISAs less than 15% cross-reactivity with rhTpo is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; ML; Myeloproliferative leukemia virus oncogene ligand; Thrombopoietin
基因别名: Mgdf; Ml; Mpllg; Thpo; Tpo
UniProt ID: (Rat) P49745, (Mouse) P40226
Entrez Gene ID: (Rat) 81811, (Mouse) 21832
positive regulation of protein phosphorylation
cell proliferation
positive regulation of cell proliferation
myeloid cell differentiation
response to lipopolysaccharide
thrombopoietin-mediated signaling pathway
positive regulation of megakaryocyte differentiation
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
