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Thrombospondin-1 Antibody (14-9756-82) in IHC (P)
Immunohistochemistry of formlin-fixed paraffin embedded human liver tissue using 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Thrombospondin-1 Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-9756-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
A6.1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572988
产品详细信息
Description: The A6.1 monoclonal antibody recognizes human, mouse, and rat thrombospondin-1, a member of the thrombospondin family of extracellular calcium-binding proteins. Thrombospondin-1 is a secreted glycoprotein that was first discovered in activated platelets but is also expressed by endothelial cells, adipocytes, fibroblasts, macrophages, monocytes, and smooth muscle cells. Thrombospondin-1 functions by binding members of the extracellular matrix such as fibronectin, integrins, and heparin, and its signaling is mediated through activation of the TGF beta signaling pathway. Thrombospondin-1 has been shown to be involved in angiogenesis, inflammation, osteogenesis, cell proliferation, and synaptogenesis during neural development.
Applications Reported: This A6.1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry.
Applications Tested: This A6.1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 µg/mL. This A6.1 antibody has been tested by immunocytochemistry of formaldehyde or methanol fixed and permeabilized human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Glycoprotein G; Thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1
基因别名: tbsp1; THBS; THBS-1; THBS1; TSP; TSP-1; TSP1
UniProt ID: (Human) P07996
Entrez Gene ID: (Human) 7057, (Rat) 445442, (Mouse) 21825
phosphatidylserine binding
glycoprotein binding
fibronectin binding
integrin binding
calcium ion binding
protein binding
heparin binding
fibroblast growth factor binding
low-density lipoprotein particle binding
identical protein binding
laminin binding
proteoglycan binding
transforming growth factor beta binding
fibrinogen binding
collagen V binding
extracellular matrix binding
activation of MAPK activity
response to hypoxia
negative regulation of endothelial cell proliferation
negative regulation of cell-matrix adhesion
sprouting angiogenesis
chronic inflammatory response
platelet degranulation
negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
negative regulation of dendritic cell antigen processing and presentation
inflammatory response
immune response
response to unfolded protein
cell cycle arrest
cell adhesion
response to glucose
positive regulation of endothelial cell migration
negative regulation of endothelial cell migration
negative regulation of plasma membrane long-chain fatty acid transport
negative regulation of nitric oxide mediated signal transduction
negative regulation of cGMP-mediated signaling
negative regulation of plasminogen activation
positive regulation of macrophage chemotaxis
positive regulation of fibroblast migration
cell migration
negative regulation of angiogenesis
peptide cross-linking
positive regulation of blood coagulation
extracellular matrix organization
positive regulation of cell migration
positive regulation of transforming growth factor beta receptor signaling pathway
regulation of cGMP metabolic process
response to magnesium ion
response to progesterone
negative regulation of interleukin-12 production
positive regulation of transforming growth factor beta1 production
cellular response to heat
response to endoplasmic reticulum stress
protein O-linked fucosylation
negative regulation of fibroblast growth factor receptor signaling pathway
positive regulation of phosphorylation
response to drug
positive regulation of tumor necrosis factor biosynthetic process
positive regulation of macrophage activation
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of blood vessel endothelial cell migration
negative regulation of blood vessel endothelial cell migration
engulfment of apoptotic cell
positive regulation of translation
positive regulation of angiogenesis
behavioral response to pain
positive regulation of chemotaxis
response to calcium ion
negative regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of fibrinolysis
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of endothelial cell apoptotic process
positive regulation of reactive oxygen species metabolic process
negative regulation of endothelial cell chemotaxis
negative regulation of extrinsic apoptotic signaling pathway
outflow tract morphogenesis
endocardial cushion development
growth plate cartilage development
response to mechanical stimulus
positive regulation of cell-substrate adhesion
response to testosterone
blood vessel morphogenesis
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
positive regulation of transforming growth factor beta production
cellular response to nitric oxide
