Invitrogen

Thymidylate Synthase Monoclonal Antibody (SP112)

产品信息

MA5-16405

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:100

流式细胞分析 (Flow)

1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

SP112

抗原

Synthetic peptide near C-terminus of human TS.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.113 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.2, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2537924

产品详细信息

Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma or tonsil tissue.

靶标信息

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

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