Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Thymidylate Synthase Monoclonal Antibody (SP112)
Product Details
MA5-16405
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP112
Immunogen
Synthetic peptide near C-terminus of human TS.
Conjugate
Form
Liquid
Concentration
0.113 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537924
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma or tonsil tissue.
Target Information
Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: MGC88736; Thymidylate synthase; TS; TSase; tysy
Gene Aliases: HST422; OK/SW-cl.29; TMS; TS; TYMS
UniProt ID: (Human) P04818
Entrez Gene ID: (Human) 7298
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
nucleobase-containing compound metabolic process
dTMP biosynthetic process
dTTP biosynthetic process
aging
circadian rhythm
cell proliferation
deoxyribonucleoside monophosphate biosynthetic process
response to toxic substance
cell growth
immortalization of host cell by virus
uracil metabolic process
organ regeneration
methylation
response to progesterone
response to vitamin A
response to cytokine
tetrahydrofolate interconversion
response to drug
response to ethanol
dUMP metabolic process
pyrimidine nucleoside biosynthetic process
response to organophosphorus
developmental growth
cartilage development
response to glucocorticoid
response to folic acid
intestinal epithelial cell maturation
DNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support