Invitrogen

Transferrin Receptor Polyclonal Antibody

Transferrin Receptor Antibody in Immunocytochemistry (ICC/IF)

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Transferrin Receptor Antibody (PA5-27739) in ICC/IF

Transferrin Receptor Polyclonal Antibody detects CD71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD71 protein stained by Transferrin Receptor Polyclonal Antibody (Product # PA5-27739) diluted at 1:500. Blue: Hoechst 33342 staining.

Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-27739

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

- -

免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 169 and 407 of Human CD71

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.47 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545215

产品详细信息

Recommended positive controls: Jurkat, Raji, NCI-H929, K562, HL-60, NIH3T3.

Predicted reactivity: Dog (81%), Cat (86%), Pig (82%), Rhesus Monkey (94%), Bovine (84%), Guinea pig (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The LRRK2 signaling network converges on a centriolar phospho-Rab10/RILPL1 complex to cause deficits in centrosome cohesion and cell polarization.

Biology open

Lara Ordóñez AJ,Fasiczka R,Fernández B,Naaldijk Y,Fdez E,Blanca Ramírez M,Phan S,Boassa D,Hilfiker S

PA5-27739 was used in Immunocytochemistry to reveal a common molecular pathway by which enhanced LRRK2 kinase activity impacts upon centrosome-related events to alter the normal biology of a cell.

Mon Aug 15 00:00:00 UTC 2022

Anthracyclins Increase PUFAs: Potential Implications in ER Stress and Cell Death.

Cells

Balgoma D,Kullenberg F,Calitz C,Kopsida M,Heindryckx F,Lennernäs H,Hedeland M

PA5-27739 was used in Immunohistochemistry (Paraffin) to investigate if the modulation of different lipid metabolic pathways may be considered for generalized and personalized metabochemotherapies.

Tue May 11 00:00:00 UTC 2021

Anthracyclins Increase PUFAs: Potential Implications in ER Stress and Cell Death.

Cells

Balgoma D,Kullenberg F,Calitz C,Kopsida M,Heindryckx F,Lennernäs H,Hedeland M

PA5-27739 was used in Immunohistochemistry (Paraffin) to investigate if the modulation of different lipid metabolic pathways may be considered for generalized and personalized metabochemotherapies.