Invitrogen

Transferrin Receptor Monoclonal Antibody (DF1513)

Transferrin Receptor Antibody in Immunocytochemistry (ICC/IF)

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Transferrin Receptor Antibody (MA5-16643) in ICC/IF

Immunofluorescence analysis of Transferrin Receptor was performed using 70% confluent log phase HeLa cells treated with BrefeldinA (5 µg/mL for 30 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Transferrin Receptor Monoclonal Antibody (Product # M... View More

Transferrin Receptor Antibody in Flow Cytometry (Flow)

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产品信息

MA5-16643

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:250

流式细胞分析 (Flow)

1:10-1:25

产品规格

种属反应

Human, Mustelid, Rhesus monkey

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

DF1513

抗原

KG1 cell line

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538138

产品详细信息

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a approximately 190 kDa homodimeric glycoprotein expressed by proliferating cells.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。