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Troponin I Antibody (701585) in ICC/IF
Immunofluorescent analysis of Troponin I was done on Primary Human Cardiomyocytes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Troponin I Recombinant Rabbit Monoclonal Antibody (Product # 701585) at a dilution of 1:400 in 1% BSA and incubated for 3 hour... View More
产品信息
701585
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1H11L19
抗原
human Troponin I
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532494
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); DKFZp451O223; Tpn1; troponin 1, type 3; troponin I type 1 (skeletal, slow); troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I, skeletal, slow 1; Troponin I, slow isoform; Troponin I, slow skeletal muscle; Troponin I, slow-twitch isoform
基因别名: 2700018B22Rik; AI747285; CMD1FF; CMD2A; CMH7; Ctni; RCM1; SSTNI; Tn1; TnI; TNN1; TNNC1; TNNI1; TNNI3
UniProt ID: (Human) P19237, (Human) P19429, (Mouse) Q9WUZ5, (Rat) P13413, (Rat) P23693, (Mouse) P48787
Entrez Gene ID: (Human) 7135, (Human) 7137, (Mouse) 21952, (Rat) 29388, (Rat) 29248, (Mouse) 21954
skeletal muscle contraction
regulation of striated muscle contraction
transition between fast and slow fiber
muscle filament sliding
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
vasculogenesis
regulation of systemic arterial blood pressure by ischemic conditions
cellular calcium ion homeostasis
regulation of smooth muscle contraction
heart development
negative regulation of ATPase activity
heart contraction
regulation of cardiac conduction
regulation of muscle contraction
striated muscle contraction
