Invitrogen

Troponin I Recombinant Superclonal™ Antibody (1HCLC)

Troponin I Antibody in Immunocytochemistry (ICC/IF)

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Troponin I Antibody (710580) in ICC/IF

Immunofluorescent analysis of Troponin I was done on Primary Human Cardiomyocytes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Troponin I Recombinant Rabbit Superclonal™ Antibody (Product # 710580) at a dilution of 1:400 in 1% BSA and incubated for 3 hou... View More

Troponin I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Troponin I Antibody in Western Blot (WB)

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产品信息

710580

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

1HCLC

抗原

human Troponin I

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532743

产品详细信息

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); DKFZp451O223; Tpn1; troponin 1, type 3; troponin I type 1 (skeletal, slow); troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I, skeletal, slow 1; Troponin I, slow isoform; Troponin I, slow skeletal muscle; Troponin I, slow-twitch isoform

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基因别名: 2700018B22Rik; AI747285; CMD1FF; CMD2A; CMH7; Ctni; RCM1; SSTNI; Tn1; TnI; TNN1; TNNC1; TNNI1; TNNI3