Invitrogen

UBC9 Polyclonal Antibody

UBC9 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

UBC9 Antibody (PA5-19609) in ICC/IF

Immunofluorescence analysis of UBC9 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with UBC9 Polyclonal Antibody (Product # PA5-19609) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

Product Image Gallery

UBC9 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models and tissues tested owing to their inherent genetic constitution. Higher expression of UBC9 was observed in MCF7, 3T3-L1, Mouse Spleen, Rat Spleen, Mouse Thymus in comparison to BJ, 3T3-L1 differentiated to adipocytes and Mouse Heart using Anti-UBC9 polyclonal antibody (Product # PA5-19609) in Western Blot. (doi: 10.1210/en.2010-0417) {RE}

产品信息

PA5-19609

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

1 µg/mL

免疫沉淀 (IP)

5 µL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human UBE2I / UBC9.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with <1mg/mL BSA

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_10987506

产品详细信息

This antibody is predicted to react with chicken, Xenopus laevis and zebrafish based on sequence homology.

靶标信息

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: mUBC9; OTTHUMP00000198131; p18; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9); Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); Ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I

查看更多收起

基因别名: 5830467E05Rik; C358B7.1; F830028O17Rik; P18; UBC9; UbcE2A; Ubce2i; UBCE9; UBE2I

查看更多收起

UniProt ID: (Human) P63279, (Rat) P63281, (Mouse) P63280

查看更多收起

Entrez Gene ID: (Human) 7329, (Rat) 25573, (Mouse) 22196

查看更多收起

功能