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UBE2D1 Antibody (PA5-102532) in ICC/IF
Immunocytochemistry analysis of UBE2D1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with UBE2D1 polyclonal antibody (Product # PA5-102532) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-102532
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human UBE2D1(Accession P51668), corresponding to amino acid residues V102-M147.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2851934
产品详细信息
Antibody detects endogenous levels of total UBE2D1.
靶标信息
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: (E3-independent) E2 ubiquitin-conjugating enzyme D1; E2 ubiquitin-conjugating enzyme D1; E2(17)KB 1; SFT; Stimulator of Fe transport; UBC 4/5; UBC4/5 homolog; UBCH 5; UBCH 5A; UbcH5; ubiquitin; Ubiquitin carrier protein D1; ubiquitin conjugating enzyme E2D 1; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog; Ubiquitin-protein ligase D1
基因别名: E2(17)KB1; SFT; UBC4/5; UBC5A; UBCH5; UBCH5A; UBE2D1
UniProt ID: (Human) P51668, (Mouse) P61080, (Rat) D3ZDK2
Entrez Gene ID: (Human) 7321, (Mouse) 216080, (Rat) 361831
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
ubiquitin-dependent protein catabolic process
BMP signaling pathway
anaphase-promoting complex-dependent catabolic process
positive regulation of protein ubiquitination
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of transcription from RNA polymerase II promoter in response to hypoxia
protein K48-linked ubiquitination
