Invitrogen

UHRF1 Polyclonal Antibody

UHRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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UHRF1 Antibody (PA5-27969) in IHC (P)

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using UHRF1 (Product # PA5-27969) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

UHRF1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

UHRF1 Antibody — Fig. 6 LASP-1 associates with epigenetic proteins UHRF1, DNMT1, G9a and Snail1 A) LASP-1 co-immunoprecipitates with UHRF1 and DNMT1. 100 mug of the nuclear extracts from MDA-Bone-Un cells stimulated with vehicle (0 min) or 500 ng /ml of CXCL12 for 15 min were incubated with control mouse monoclonal IgG (Mock) or with mouse anti-LASP-1 antibody. LASP1-bound proteins were eluted and analyzed for endogenous LASP-1 and associated proteins UHRF1 and DNMT1. Bottom panel: 15 mug of total cell lysates from MDA-Bone-Un cells that were stimulated with vehicle (0 min) or CXCL12 for 15 min. LASP-1 lysate blot was obtained after stripping and re-probing for LASP-1 after the detection of Snail1 first. The experiment was repeated twice and the representative blot was quantified and shown. NS - Non-silenced control; KD - LASP-1 knock down. The band intensities of proteins were quantified by using Image J analysis and normalized to their respective non-stimulated control (bands in -CXCL12 lane). The fold change was given above the bands to track the level of the immunoprecipitated proteins with respect to their protein levels in the lysates. B) LASP-1 co-immunoprecipitates with G9a and Snail1. 100 mug of the nuclear extracts from MDA-Bone-Un cells stimulated with vehicle (0 min and Mock) or 500 ng /ml of CXCL12 for 15 min were incubated with control mouse monoclonal IgG (Mock) or with mouse anti-LASP-1 antibody. Bound proteins were eluted and analyzed for endogenous LASP-1 and associated G9a {TM}

产品信息

PA5-27969

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 51 and 516 of Human UHRF1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545445

产品详细信息

Recommended positive controls: HeLa.

Predicted reactivity: Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MiR-9-1 Suppresses Cell Proliferation and Promotes Apoptosis by Targeting UHRF1 in Lung Cancer.

Technology in cancer research & treatment

Jia CY,Xiang W,Liu JB,Jiang GX,Sun F,Wu JJ,Yang XL,Xin R,Shi Y,Zhang DD,Li W,Zuberi Z,Zhang J,Lu GX,Wang HM,Wang PY,Yu F,Lv ZW,Ma YS,Fu D

Published figure using UHRF1 polyclonal antibody (Product # PA5-27969) in Immunohistochemistry

Mon Jan 24 00:00:00 UTC 2022

LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex.

Oncogene

Duvall-Noelle N,Karwandyar A,Richmond A,Raman D

PA5-27969 was used in western blot to study the relationship between nuclear LASP-1 and the epigenetic machinery in breast cancer

Thu Mar 03 00:00:00 UTC 2016

生物信息学

蛋白别名: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; hUHRF1; HuNp95; Inverted CCAAT box-binding protein of 90 kDa; liver regeneration-related protein LRRG126; MGC138707; mUhrf1; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; Transcription factor ICBP90; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; ubiquitin-like, containing PHD and RING finger domains, 1; Ubiquitin-like-containing PHD and RING finger domains protein 1

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基因别名: Ac2-121; AL022808; hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1

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UniProt ID: (Human) Q96T88, (Mouse) Q8VDF2

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Entrez Gene ID: (Human) 29128, (Mouse) 18140, (Rat) 316129

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功能