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UHRF1 Antibody (PA5-88643) in WB
Western blot analysis of UHRF1 in mouse brain. Samples were incubated with UHRF1 Polyclonal antibody (Product # PA5-88643) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
产品信息
PA5-88643
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 139-298 of human UHRF1 (NP_0374143)
偶联物
形式
Liquid
浓度
8.92 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805049
产品详细信息
Immunogen sequence: MWDETELGLY KVNEYVDARD TNMGAWFEAQ VVRVTRKAPS RDEPCSSTSR PALEEDVIYH VKYDDYPENG VVQMNSRDVR ARARTIIKWQ DLEVGQVVML NYNPDNPKER GFWYDAEISR KRETRTAREL YANVVLGDDS LNDCRIIFVD EVFKIERPGE; Positive Samples: Mouse brain; Cellular Location: Nucleus
靶标信息
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; MGC138707; mUhrf1; Nuclear protein 95; Nuclear zinc finger protein Np95; RING-type E3 ubiquitin transferase UHRF1; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; Ubiquitin-like-containing PHD and RING finger domains protein 1
基因别名: AL022808; ICBP90; Np95; RNF106; Uhrf1
UniProt ID: (Mouse) Q8VDF2
Entrez Gene ID: (Mouse) 18140
core promoter proximal region sequence-specific DNA binding
DNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
methyl-CpG binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
nucleosomal histone binding
methylated histone binding
histone binding
identical protein binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
hemi-methylated DNA-binding
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
cell cycle
cell proliferation
maintenance of DNA methylation
histone monoubiquitination
chromatin modification
histone ubiquitination
positive regulation of cellular protein metabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
protein autoubiquitination
regulation of methylation-dependent chromatin silencing
