Invitrogen

ULK1 Recombinant Rabbit Monoclonal Antibody (JA58-36)

ULK1 Antibody in Immunocytochemistry (ICC/IF)

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ULK1 Antibody (MA5-32699) in ICC/IF

Immunofluorescence analysis of ULK1 was performed using 70% confluent log phase Jurkat and Ramos cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with ULK1 Recombinant Rabbit Monoclonal Antibody (JA58-36) (Product # MA5-32699) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fl... View More

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-32699

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JA58-36

抗原

Synthetic peptide within Human ULK1 aa 930-979

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809976

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1

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基因别名: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1

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UniProt ID: (Human) O75385, (Mouse) O70405

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Entrez Gene ID: (Human) 8408, (Rat) 360827, (Mouse) 22241

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