Invitrogen

ULK2 Polyclonal Antibody

ULK2 Antibody in Immunocytochemistry (ICC/IF)

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ULK2 Antibody (PA5-22173) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ULK2 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ULK2 Polyclonal Antibody (Product # PA5-22173) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

ULK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-22173

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 693 and 968 of ULK2 (Uniprot ID#Q8IYT8)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.24 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153516

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1, NIH-3T3.

Predicted reactivity: Mouse (94%), Rat (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Remodeling of ER-exit sites initiates a membrane supply pathway for autophagosome biogenesis.

EMBO reports

Ge L,Zhang M,Kenny SJ,Liu D,Maeda M,Saito K,Mathur A,Xu K,Schekman R

PA5-22173 was used in Western Blot to indicate a pathway wherein FIP200 and CTAGE5 facilitate starvation-induced remodeling of the ERES, a prerequisite for the production of COPII vesicles budded from the ERGIC that contribute to autophagosome formation.