Invitrogen

UNG Polyclonal Antibody

UNG Antibody in Immunocytochemistry (ICC/IF)

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UNG Antibody (PA5-89032) in ICC/IF

Immunofluorescence analysis of UNG in HeLa cells. Samples were incubated with UNG Polyclonal antibody (Product # PA5-89032) using a dilution of 1:100. Blue: DAPI for nuclear staining.

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产品信息

PA5-89032

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:50-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UNG (NP_5504331)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.17 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2805309

产品详细信息

Immunogen sequence: MIGQKTLYSF FSPSPARKRH APSPEPAVQG TGVAGVPEES GDAAAIPAKK APAGQEEPGT PPSSPLSAEQ LDRIQRNKAA ALLRLAARNV PVGFGESWKK; Positive Samples: Mouse skeletal muscle, Mouse heart, Rat skeletal muscle; Cellular Location: Mitochondrion, Nucleus

靶标信息

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB. The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis. Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type. Additionally, the expression of UNG is cell cycle regulated. The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells. UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。