Invitrogen

USP14 Polyclonal Antibody

USP14 Antibody in Immunocytochemistry (ICC/IF)

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USP14 Antibody (PA5-30301) in ICC/IF

Immunofluorescence analysis of Anti-USP14 Polyclonal Antibody (Product # PA5-30301) was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with USP14 Polyclonal Antibody (Product # PA5-30301) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondar... View More

USP14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

USP14 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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USP14 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with USP14 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-USP14 Polyclonal Antibody (Product # PA5-30301). {KD}

产品信息

PA5-30301

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 67 and 325 of Human USP14

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547775

产品详细信息

Recommended positive controls: HeLa, A431, mouse brain, rat brain.

Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (88%), Rabbit (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ataxia; Deubiquitinating enzyme 14; dUB-type TGT for deubiquitinating enzyme; tRNA-guanine transglycosylase, 60-kD subunit; Ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14; ubiquitin specific protease 14 (tRNA-guanine transglycosylase); Ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14

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基因别名: 2610005K12Rik; 2610037B11Rik; AW107924; ax; C78769; nmf375; TGT; USP14

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UniProt ID: (Human) P54578, (Mouse) Q9JMA1

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Entrez Gene ID: (Human) 9097, (Mouse) 59025, (Rat) 291796

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